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Detecting different RNAs in the same cell

Date: 2022-11-14

In biological study, one sometimes needs to detect the simultaneous presence of a micro RNA (miRNA) and its putative target mRNA in the same cell. As no such technique is unavailable, Dr. Wen-Hsiung Li’s team at Biodiversity Research Center develop a in situ method for this purpose. It uses rolling-circle amplification of RNAs to achieve a high signal/noise ratio. It can be applied to simultaneous detection of multiple RNAs in the same cell. The results were published in Plant Biotechnology Journal.

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