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Abstract

Understanding the influence of oxygen on cellular functions and behaviors is crucial for investigating various physiological and pathological conditions. To study cellular responses under specific oxygen microenvironments, various advanced in vitro cell culture models have been developed. However, precise manipulation of oxygen microenvironments and accurate characterization of the oxygen variations with great spatiotemporal resolutions remain challenging. In this talk, I will discuss the approaches developed in my lab to manipulate the oxygen microenvironments for cell culture using microfluidic devices, and the oxygen sensing scheme based on frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) for oxygen microenvironment characterization on organoid and microfluidic cell culture models.