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   Huang, Kai-Fa
 

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E-mail: huangkf@gate.sinica.edu.tw
Telephone: 02-2785-5696 ext.5030

* EDUCATION AND POSITIONS HELD

  • B.S., Department of Veterinary Medicine, National Chung Hsing University, 1991

  •  M.S., Institute of Biochemical Sciences, National Taiwan University, 1993

  •  Research Assistant, Institute of Biological Chemistry, Academia Sinica, 1993-present

  •  Ph.D. Student, Institute of Biochemical Sciences, National Taiwan University, 2002-present

* HONORS

  • Research Award, National Science Council, 1994

  • Research Award, National Science Council, 1999

  • Outstanding Performance of Ph.D. Research, Institute of Biochemical Sciences, National Taiwan University, 2004

  • Service Award, Academia Sinica, 2004

* RESEARCH INTERESTS

  • Structure, function and regulation of snake venom proteins that are interesting and important in pharmacology.

  • Structure, catalysis mechanism, inhibition and tissue distribution of human glutaminyl cyclase, an enzyme responsible for protein N-terminal pyroglutamate formation.

  • Assembly of uroplakin particle, a four-membrane-protein complex on the urothelial surface of mammals.

* SELECTED PUBLICATIONS

  • Huang, K.-F., Liu, Y.-L., Cheng, W.-J., Ko, T.-P. & Wang, A. H.-J. (2005) Crystal structures of human glutaminyl cyclase, an enzyme responsible for protein N-terminal pyroglutamate formation. Proc. Natl. Acad. Sci. USA. 102: 13117-13122.

  • Huang, K.-F., Liu, Y.-L. & Wang, A. H.-J. (2005) Cloning, expression, characterization, and crystallization of a glutaminyl cyclase from human bone marrow: A single zinc metalloenzyme. Protein Expr. Purif. 43: 65-72.

  • Huang, K.-F., Ko, T.-P., Hung, C.-C., Chu, J., Wang, A. H.-J. & Chiou, S.-H. (2004) Crystal structure of a platelet-agglutinating factor isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. J. 378: 399-407.

  • Huang, K.-F., Chiou, S.-H., Ko, T.-P., Yuann, J.-M. & Wang, A. H.-J. (2002) The 1.35 A structure of cadmium-substituted TM-3, a snake-venom metalloproteinase from Taiwan habu: elucidation of a TNFa-converting enzyme-like active-site structure with a distorted octahedral geometry of cadmium. Acta Crystallogr. D Biol Crystallogr. 58: 1118-1128.

  • Huang, K.-F., Chiou, S.-H., Ko, T.-P. & Wang, A.H.-J. (2002) Determinants of the inhibition of a Taiwan habu venom metalloproteinase by its endogenous inhibitors revealed by X-ray crystallography and synthetic inhibitor analogues. Eur. J. Biochem. 269: 3047-3056.

  • Huang, K.-F., Chow, L.-P. & Chiou, S.-H. (1999) Isolation and characterization of a novel proteinase inhibitor from the snake serum of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. Biophys. Res. Commun. 263: 610-616.

  • Huang, K.-F., Hung, C.-C., Wu, S.-H. & Chiou, S.-H. (1998) Characterization of three endogenous peptide inhibitors for multiple metalloproteinases with fibrinogenolytic activity from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. Biophys. Res. Commun. 248: 562-568.

  • Chiou, S.-H., Huang, K.-F., Chow, L.-P., Tsugita, A. & Wu, S.-H. (1996) Isolation of a venom factor devoid of proteolytic activity from Taiwan habu (Trimeresurus mucrosquamatus): N-terminal sequence homology and no functional similarity to factors IX/X-binding proteins and botrocetin. J. Protein Chem. 15: 667-674.

  • Huang, K.-F., Hung, C.-C., Pan, F.-M., Chow, L.-P., Tsugita, A. & Chiou, S.-H. (1995) Characterization of multiple metalloproteinases with fibrinogenolytic activity from the venom of Taiwan habu (Trimeresurus mucrosquamatus): protein microsequencing coupled with cDNA sequence analysis. Biochem. Biophys. Res. Commun. 216: 223-233.

  • Hung, C.-C., Huang, K.-F. & Chiou, S.-H. (1994) Characterization of one novel venom protease with b-fibrinogenase activity from the Taiwan habu (Trimeresurus mucrosquamatus): purification and cDNA sequence analysis. Biochem. Biophys. Res. Commun. 205: 1707-1715.

  • Huang, K.-F., Hung, C.-C. & Chiou, S.-H. (1993) Characterization of three fibrinogenolytic proteases isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. Mol. Biol. Int. 31: 1041-1050.

  • Chang, C.-C., Huang, T.-Y., Kuo, K.-W., Chen, S.-W., Huang, K.-F. & Chiou, S.-H. (1993) Sequence characterization of a novel a-neurotoxin from the king cobra (Ophiophagus hannah) venom. Biochem. Biophys. Res. Commun. 91: 214-223.

  • Chiou, S.-H., Hung, C.-C. & Huang, K.-F. (1992) Characterization of a protease with a- and b-fibrinogenase activity from the Western diamondback rattlesnake, Crotalus atrox. Biochem. Biophys. Res. Commun. 187: 389-396.
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